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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be noticed in many tumors, which ends up in chromosome instability, and thus might add to tumorigenesis. This protein was located to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital job in marketing mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two more splice variants have been described but detailed nucleotide sequence details will not be nevertheless accessible.

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Blocking peptides are peptides that bind precisely on the target antibody and block antibody binding. These peptide generally has the epitope recognized with the antibody.

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This tutorial outlines further more attainable optimization of mobile and immunoassay parameters to make sure the best possible final results are...

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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the very best effects are...

Antibodies sure to the blocking peptide now not bind to your epitope to the focus on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is specific.

Target Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterised by a spectacular periodicity in protein abundance in the mobile cycle. Cyclins function as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E varieties a complex with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary and is also degraded as cells development as a result of S section.

The measurement of website protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...

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